Potato Cyst Nematode Technical Working Group

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Public documents

National Survey

1. Survey, Detection, and Identification Flow Chart

Globodera pallida Host List

1. Host range list prepared by M. Sullivan, National Weed Management Laboratory, Fort Collins, CO.

Appendix 1 of PCN TWG Meeting Report, Boise Idaho, May 10-11, 2006

1. Armstrong, M. R., Blok, V. C., Phillips, M. S., Trudgill, D. L. 1998. The novel mitochondrial genome architecture of the potato cyst nematode Globodera pallida. Scottish Crop Research Institute 1998/99 Report, 150-152.
2. Been, T. H., Schomaker, C. H. 2000. Development and evaluation of sampling methods for fields with infestation foci of potato cyst nematodes (Globodera rostochiensis and G. pallida). Phytopathology 90, 647-656.
3. EPPO Quarantine Pest.  Data Sheets on Quarantine Pests.Globodera rostochiensis and Globodera pallida.
4. EPPO Standards. Diagnostic protocols for regulated pests, Globodera rostochiensis and Globodera pallida. 2004 Bulletin OEPP/EPPO Bulletin 34, 309-314.
5. Evans, K., Webster, R., Barker, A., Halford, P., Russell, M., Stafford, J., Griffin, S. 2003. Mapping infestations of potato cyst nematodes and the potential for spatially varying application of nematicides. Precision Agriculture 4, 149-162.
6. Ibrahim, S. K., Minnis, S. T., Barker, A. D. P., Russell, M. D., Haydock, P. P. J., Evans, K., Grove, I. G., Woods, S. R., Wilcox, A. 2001. Evaluation of PCR, IEF, and ELISA techniques for the detection and identification of potato cyst nematodes from field soil samples in England and Wales. Pest Management Science 57, 1068-1074.
7. Investigation of potato cyst nematode control. Rothamsted Research. 2003. Reviewed by B. Kerry, A. Barker, K. Evans. Nematode Interactions Unit, Plant-Pathogens Interactions Division, Rothamsted Research, Harpenden, Herts. 92 pp.
8. Lilley, C. J., Atkinson, H. J., Urwin, P. E. 2005. Molecular aspects of cyst nematodes. Molecular Plant Pathology 6, 577-588.
9. Perry, J. N. Measures of spatial pattern for counts. 1998. Ecology 79, 1008-1017.
10. Pylypenko, L. A., Uehara, T., Phillips, M. S., Sigareva, D. D., Blok, V. C. 2005. Identification of Globodera rostochiensis and G. pallida in the Ukraine by PCR. European Journal of Plant Pathology 111, 39-46.
11. Reid, A., Pickup, J. 2005. Molecular characterization of a morphologically unusual potato cyst nematode. 2005 OEPP/EPPO, Bulletin 35, 69-72.
12. The Potato Cyst Nematode (Globodera rostochiensis). A pest of national importance.  USDA-ARS/Cornell University.
13. Trudgill, D. L., Elliott, M. J., Evans, K., Phillips, M. S.  2003.  The white potato cyst nematode (Globodera pallida) – a critical analysis of the threat in Britain.  Annals of Applied Biology 143, 73-80. 
14. USDA Golden Nematode Program Manual.  2006 Interim Edition. 

Presentations from PCN TWG Meeting in Boise Idaho, May 10-11, 2006

1. Nematode Surveys under the Cooperative Agriculture Pest Survey (CAPS), Tim McNary, USDA APHIS PPQ Western Region, Senior Regional Program Manager
2. USDA-ARS Identification of Potato Cyst Nematodes in the United States.  Zafar Handoo and Lynn Carta, USDA ARS Nematology Lab
3. Golden Nematode Program, History, Successes, Lessons Learned.  Dan Kepich, USDA APHIS PPQ
4. The UK/EU experience.  Statutory control measures: The EU PCN Directive.  Mark Phillips, Scottish Crop Research Institute.

Protocols for processing of soil samples for PCN

1. Fenwick Can
2. USDA Cyst Extractor
3. Centrifugation and Sugar Flotation
4. Elutriator plus Sugar Flotation
5. Elutriator plus Acetone Extraction

Washing

1. Effect of washing on the removal of potato cyst nematode from potato tubers grown on Globodera pallida infested soil.

Sampling

1. Consolidated information on PCN sampling primarily from the UK and the EU.
   • A table of sampling methods and selected information regarding sampling for PCN based mostly on the experience in the UK and The Netherlands.

Management

1. Biological and Cultural Controls
   • Trap cropping, fungal and bacterial biological controls.

Composting

1. Noble, R., Roberts, S.J.  2003.  A Review of the Literature on Eradication of Plant Pathogens and Nematodes during Composting, Disease Suppression and Detection of Plant Pathogens in Compost.  Standards, Research Report.  The Waste and Resources Action Programme.
2. Noble, R., Roberts, S.J.  2004.  Eradication of plant pathogens and nematodes during composting: a review.  Plant Pathology, 53, 548-568.
3. Olsen, N., Nolte, P., Harding, G., Ohlensehlen, B.  2001.  Cull and Waste Potato Management.  University of Idaho, College of Agriculture, Cooperative Extension System.  CIS 814.

Biofumigation

1. Biofumigant crops related to management of PCN
   • Collected information on the use of crops as biofumigants to reduce or manage nematode populations.